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The IBEX Sciences Barium Chloride 10% Solution (w/v) is a ready-to-use inorganic chemical reagent utilized heavily in clinical biochemistry (urinalysis) and analytical testing. Prepared by dissolving 10 grams of pure barium chloride (BaCl₂) into distilled water to make a total volume of 100 mL, this solution acts as a precise analytical tool primarily for precipitation reactions. [1, 2, 3, 4]
Core Laboratory Applications
- Urinalysis for Bile Pigments (Schlesinger’s / Fouchet’s Test):
In clinical diagnostics, Barium Chloride 10% is used to detect the presence of bilirubin (bile pigment) in urine. When added to a urine specimen, the barium ions combine with the natural sulfate radicals present in the urine, generating a heavy precipitate of barium sulfate (BaSO₄).- Any bilirubin present in the sample adheres tightly to these large, precipitating barium molecules.
- When Fouchet’s reagent (containing trichloroacetic acid and ferric chloride) is applied to the sediment, it oxidizes the trapped yellow bilirubin into green biliverdin, confirming a positive test for liver or gallbladder anomalies. [1, 2]
- Sulfate Quantification:
Outside of clinical contexts, the solution is widely deployed in analytical chemistry to identify or quantify sulfate ions (SO₄²⁻) in unknown compounds, wastewater, or soil extracts. The instant formation of an insoluble white precipitate confirms the presence of sulfates. [1, 2, 3, 4]
Reagent Specifications & Chemistry
- Concentration: 10% weight/volume (w/v) aqueous solution.
- Appearance: Completely clear, colorless, and odorless liquid.
- pH Level: Typically highly acidic (~0.8 to 1.0) depending on stabilization parameters.
- Miscibility: Completely miscible in water. [1, 2, 3, 4]
Critical Safety & Handling Precautions
- Acute Oral Toxicity: Barium chloride is highly toxic if swallowed. It acts as a systemic muscle stimulant and poison that can affect cardiac function. Oral pipetting must never be performed; use automated pipettes or bulbs. [1, 2, 3, 4, 5]
- First Aid Matrix:
- If Swallowed: Immediately rinse the mouth out with water and seek prompt medical attention.
- Skin/Eye Contact: Flush the area thoroughly with clean running water for at least 15 minutes. [, 2]
- PPE Requirements: Standard laboratory protocols require chemical splash goggles, a lab coat, and protective nitrile gloves. [1]
- Storage Environment: Store tightly sealed in a secure chemical locker at room temperature (15°C–30°C), safely segregated from food products, strong acids, and oxidizing agents. [1, 2, 3]
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