(+92) 320 0038000
An H&E (Hematoxylin and Eosin) Colour/Staining Kit from IBEX SCIENCES is a fundamental diagnostic and educational tool used in histology, cytology, and pathology to stain tissue sections. This staining method provides critical visual contrast, allowing researchers, pathologists, and students to clearly examine cell structures under a microscope. [1, 2, 3, 4, 5]
What is Included in the Kit?
-
- Hematoxylin Solution: The primary, nuclear stain. It binds to acidic structures (like DNA and RNA in the cell nucleus), coloring them a dark purple or blue. [1, 2, 3, 4]
- Eosin Solution: The counterstain. It binds to basic structures (like cytoplasmic proteins and extracellular matrices), coloring them varying shades of pink, orange, or red. [1, 2, 3, 4]
- Bluing/Differentiating Solutions: Depending on the exact kit formulation, ancillary solutions are included to adjust the contrast and firmly lock the blue hue of the hematoxylin before counterstaining with eosin. [1, 2]
Primary Applications
-
- Histological Oversight: Fast and reliable oversight staining of paraffin-embedded or frozen tissue sections.
- Medical Training: Extensively used alongside medical teaching models and science lab equipment for educational laboratories.
- Pathology Checkpoints: Distinguishing between normal and abnormal structures in tumor biopsies or tissue anomalies. [1, 2, 3, 4, 5]
General Staining Workflow
While exact timing varies by protocol and sample thickness, the general procedure follows these steps: [1]
- Deparaffinization & Rehydration: If using paraffin sections, tissue slides must be cleared and brought down to water.
- Hematoxylin Staining: Slides are immersed in Hematoxylin to stain cell nuclei blue/purple.
- Washing & Bluing: Excess stain is rinsed away, and a bluing reagent is applied to shift the hematoxylin color to a crisp blue.
- Eosin Counterstaining: Slides are immersed in Eosin to dye the cytoplasm and connective tissue pink/red.
- Dehydration & Mounting: The slide is passed through a gradient of ethanol and clearing agents before being mounted with a coverslip for observation. [1, 2, 3, 4]
Reviews
There are no reviews yet.